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KMID : 0359319990390040772
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Korean Journal of Veterinary Research
1999 Volume.39 No. 4 p.772 ~ p.777
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A mutational anlaysis of the N-terminal protease of bovine viral diarrhea virus
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Chon Seung-Ki
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Abstract
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The uncaped genomic RNA of bovine viral diarrhea virus (BVDV) initiates translation by recruitment of eukaryotic translation initiation factors at the internal ribosome entry site (IRES). N-terminal protease (N^(pro)) is the first translation product of the open reading frame (ORF). By using the vaccinia virus SP6 RNA polymerase transient expression system, we showed previously that deletion of N^(pro) region reduced translation by 21%. To better understand the biological significance of N^(pro) for translation, we carried out a mutational analysis of the N^(pro) region of BVDV cloned in the intercistronic region of a bicistronic reporter plasmid. We constructed a bicistronic expression vector in which the entire 5 UTR and the mutated N^(pro) region ( Δ386-901, Δ415-901 and Δ657-901) was cloned between two reporter genes, chloramphenicol acetyltransferase (CAT) and luciferase (LUC). In vivo translation analyses showed that N^(pro) region was dispensible for efficient translation. The results indicate that the N^(pro) region is not essential for BVDV RNA translation and the 3´ boundary of BVDV IRES is expanded into N^(pro) region, suggesting that N^(pro) may not play a major role in BVDV replication.
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KEYWORD
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